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The binding of NEDD4 and NEDD8 was not observed when only the vector was introduced (Fig
Cryo-EM grid preparation and data collection For the cryo-EM grids preparation, 3 l of purified ghrelin-bound complex at 13 mg/ml and GHRP-6-bound ghrelin receptor complex at 8 mg/ml were applied individually onto a glow-discharged holey carbon grid (Quantifoil, Au300 R1.2/1.3) in a Vitrobot chamber (FEI Vitrobot Mark IV)
References: British Columbia Medical Association
In severely affected livers, more severe chronic lesions can be observed
