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Metabolomic investigations show that hepatocellular GSH concentrations are lower in a specific group of patients with MASLD, which is accompanied by poor production of VLDL [181]
Preclinical studies involving cell cultures and animal models were included when applicable to support mechanistic insights into NAC's effects
Determination of NAD + -dependent aldehyde dehydrogenase (ALDH) Activity ALDH activity was determined using a colorimetric assay Kit (#K731-100, BioVision, Cambridge, UK)

Long-term human safety data is still more limited than for well-established supplements like vitamin C, so periodic doctor check-ins are a reasonable precaution