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These pathways converge influencing cell fate decisions (survival vs death), proliferation rates, migration patterns, and differentiation states

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This guide cuts through the confusion by answering the critical questions: which peptides actually work nasally, how to properly administer nasal spray peptides, what dosing adjustments are needed versus injection, troubleshooting common nasal administration problems, and honestly comparing nasal versus injectable delivery effectiveness

Keywords: ulcerative colitis, GHK-Cu, SIRT1/STAT3 pathway, co-culture system, network pharmacology, molecular docking 1 Introduction Ulcerative colitis (UC) is a chronic, relapsing form of inflammatory bowel disease (IBD) characterized by immune system dysfunction and pathological overactivation of mucosal immune responses, leading to persistent inflammation and subsequent colonic mucosal injury (Raine et al., 2022)