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The culture remained at 28 C for a further 2 hours before cells were harvested by centrifugation (8000 g , 20 minutes, 4 C)
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In these models, melatonin treatment inhibited hepatic stellate cell activation, reduced matrix metalloproteinase-9 (MMP-9) activity, and significantly decreased the expression of profibrogenic factors in a dose-dependent manner [218]
Lowering levels of mitochondrial ROS (and thereby oxidative stress) successfully prevents activation and induction of these mechanisms (23)
